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Tff3 ELISA Kit (Mouse)

CAT: 0247-OKEH04680-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04680-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Trefoil factor 3, intestinal
Gene Aliases
Intestinal trefoil factor; IT; ITF; mIT; mITF; trefoil factor 3.
Gene ID
21786
Accession Number
NP_035705.1
Reactivity
Mouse, Mus musculus
Target
Trefoil factor 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tff3 ELISA Kit (Mouse) (OKEH04680) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tff3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tff3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tff3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
9 KDa
Specificity
Natural and recombinant Mouse Trefoil factor 3
Protein Length
81
NCBI Gene Symbol
Tff3
Protein Name
Q62395
Gene Name URL
Tff3
Nucleotide Accession Number
NM_011575.2

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