Products for Research Use Only
RegisterLogin

LIFR ELISA Kit (Human)

CAT: 0247-OKBB00534-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB00534-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 1147 3628 6357 1458 3817 6682 SD 65.4 152.4 419.6 97.7 221.4 487.8 CV% 5.7 4.2 6.6 6.7 5.8 7.3 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 1147 3628 6357 1458 3817 6682 Mean (pg/mL) 1147 3628 6357 1458 3817 6682 SD 65.4 152.4 419.6 97.7 221.4 487.8 SD 65.4 152.4 419.6 97.7 221.4 487.8 CV% 5.7 4.2 6.6 6.7 5.8 7.3 CV% 5.7 4.2 6.6 6.7 5.8 7.3
CAS Number
7732-18-5
Gene Name
Leukemia inhibitory factor receptor alpha
Gene Aliases
CD118, CD118 antigen, FLJ98106, FLJ99923, Leukemia inhibitory factor receptor alpha, Leukemia inhibitory factor receptor, LIF R, LIF receptor, LIF-R, Lifr, LIFR_HUMAN, SJS2, STWS, SWS
Gene ID
3977
Accession Number
NP_001121143.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q45-S833
Target
LIFR, also known as CD118 (Cluster of Differentiation 118), is a subunit of a receptor for leukemia inhibitory factor. This gene encodes a protein that belongs to the type I cytokine receptor family. It is mapped to 5p31.1. The LIF receptor (LIFR) is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of the leukemia-inhibitory factor. LIF is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, togerther with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human LIFR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LIFR has been precoated onto 96-well plates. Standards (NS0, Q45-S833) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LIFR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human LIFR amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human LIFR
NCBI Gene Symbol
LIFR
Protein Name
Leukemia inhibitory factor receptor
Gene Name URL
LIFR
Nucleotide Accession Number
NM_001127671.1

Related Products

CatalogName
OKCD02689-96WLIFR ELISA Kit (Mouse)
OKEH07429-96WLIFR ELISA Kit (Dog)
OKEH07039-96WLIFR ELISA Kit (Mouse)
OKEH06282-96WLIFR ELISA Kit (Rat)
OKCD02689LIFR ELISA Kit (Mouse) (OKCD02689)
OKCA02566LIFR ELISA Kit (Rat) (OKCA02566)
OKEH06282LIFR ELISA Kit (Rat) (OKEH06282)
OKEH07039LIFR ELISA Kit (Mouse) (OKEH07039)
OKEH07429LIFR ELISA Kit (Dog) (OKEH07429)
OKAN05905LIFR ELISA Kit (Mouse) (OKAN05905)

Alternative Products

CatalogName
ELA-E0561hHuman LIFR ELISA Kit
K4203-100LIFR (Human) ELISA Kit
352960LIFR, BioAssayTM ELISA Kit (Human)
EK1200Human LIFR PicoKine® ELISA Kit
MBS177390-01Human LIFR PicoKine ELISA Kit
GTR18830449-01Human LIFR ELISA Kit (Ready to Use)
GTR18799590Human LIFR/Lif R Alpha ELISA Kit
YP-W15595-01Human leukemia inhibitory factor receptor (LIFR) ELISA Kit
SL1057Hu-01Human leukemia inhibitory factor receptor, LIFR ELISA Kit
RE1581H-01Human LIFR (Leukemia Inhibitory Factor Receptor) ELISA Kit

Popular Products