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LIFR ELISA Kit (Mouse)

CAT: 0247-OKEH07039-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07039-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
LIF receptor alpha
Gene Aliases
A230075M04Rik; AW061234; D-factor/LIF receptor; leukemia inhibitory factor receptor; leukemia inhibitory factor receptor alpha chain; LIF; LIF-R; soluble differentiation-stimulating factor receptor.
Gene ID
16880
Accession Number
NP_001106857
Reactivity
Mouse, Mus musculus
Target
Leukemia inhibitory factor receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Lifr ELISA Kit (Mouse) (OKEH07039) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lifr has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lifr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lifr captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
6.25-400pg/mL
Recovery
Mean recovery when spiking into matrix = 97%
Sensitivity
3.215 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Leukemia inhibitory factor receptor
NCBI Gene Symbol
Lifr
Protein Name
P42703
Gene Name URL
Lifr
Nucleotide Accession Number
NM_001113386

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