LIFR ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Leukemia Inhibitory Factor Receptor (LIFR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Leukemia Inhibitory Factor Receptor (LIFR) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

LIF receptor alpha

A230075M04Rik; AW061234; D-factor/LIF receptor; leukemia inhibitory factor receptor; leukemia inhibitory factor receptor alpha chain; LIF; LIF-R; soluble differentiation-stimulating factor receptor.

16880

NP_001106857.1

Mouse|Mus musculus

Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukemia Inhibitory Factor Receptor (LIFR) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukemia Inhibitory Factor Receptor (LIFR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukemia Inhibitory Factor Receptor (LIFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leukemia Inhibitory Factor Receptor (LIFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma, tissue homogenates and other biological fluids

78-5,000pg/mL

Matrix Recovery range (%) Average (%) Serum (n=5) 78-96 92 EDTA Plasma (n=5) 82-97 87 Heparin Plasma (n=5) 80-90 83 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 78-96 92 Serum (n=5) 78-96 92 EDTA Plasma (n=5) 82-97 87 EDTA Plasma (n=5) 82-97 87 Heparin Plasma (n=5) 80-90 83 Heparin Plasma (n=5) 80-90 83

31 pg/mL

2°C to 8°C|-20°C

Component Amount Anti-LIFR Microplate 96 Wells (12 x 8 Well strips) LIFR Lyophilized Standard 2 100X Biotinylated LIFR Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-LIFR Microplate 96 Wells (12 x 8 Well strips) Anti-LIFR Microplate 96 Wells (12 x 8 Well strips) LIFR Lyophilized Standard 2 LIFR Lyophilized Standard 2 100X Biotinylated LIFR Detector Antibody 1 x 120 uL 100X Biotinylated LIFR Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

This assay has high sensitivity and excellent specificity for detection of Leukemia Inhibitory Factor Receptor (LIFR) . No significant cross-reactivity or interference between Leukemia Inhibitory Factor Receptor (LIFR) and analogues was observed.

1092

Lifr

Leukemia inhibitory factor receptor

Lifr