Products for Research Use Only
RegisterLogin

IGFBP5 ELISA Kit (Mouse)

CAT: 0247-OKBB00525-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB00525-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 1.1 3.4 6.2 1.5 3.8 6.7 SD 0.045 0.167 0.341 0.084 0.247 0.476 CV% 4.1 4.9 5.5 5.6 6.5 7.1 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 1.1 3.4 6.2 1.5 3.8 6.7 Mean (pg/mL) 1.1 3.4 6.2 1.5 3.8 6.7 SD 0.045 0.167 0.341 0.084 0.247 0.476 SD 0.045 0.167 0.341 0.084 0.247 0.476 CV% 4.1 4.9 5.5 5.6 6.5 7.1 CV% 4.1 4.9 5.5 5.6 6.5 7.1
CAS Number
7732-18-5
Gene Name
Insulin-like growth factor binding protein 5
Gene Aliases
IBP 5, IBP-5, IBP5, IBP5_HUMAN, IGF binding protein 5, IGF BP5, IGF-binding protein 5, IGFBP 5, IGFBP-5, IGFBP5, Insulin like growth factor binding protein 5, Insulin-like growth factor-binding protein 5
Gene ID
16011
Accession Number
NP_034648.2
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: L20-E271
Target
Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse IGFBP5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP5 has been precoated onto 96-well plates. Standards (NS0, L20-E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IGFBP5 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse IGFBP5
NCBI Gene Symbol
IGFBP5
Protein Name
Insulin-like growth factor-binding protein 5
Gene Name URL
IGFBP5
Nucleotide Accession Number
NM_010518.2

Related Products

CatalogName
OKEH03930-96WIGFBP5 ELISA Kit (Bovine)
OKCD08216-96WIGFBP5 ELISA Kit (Human)
OKCD08217-96WIGFBP5 ELISA Kit (Rat)
OKEH04865-96WIGFBP5 ELISA Kit (Human)
OKBB00590-96WIGFBP5 ELISA Kit (Rat)
OKEH04580-96WIgfbp5 ELISA Kit (Rat)
OKCD08216IGFBP5 ELISA Kit (Human) (OKCD08216)
OKEH03930IGFBP5 ELISA Kit (Bovine) (OKEH03930)
OKAN05434IGFBP5 ELISA Kit (Human) (OKAN05434)
OKBB00590IGFBP5 ELISA Kit (Rat) (OKBB00590)

Alternative Products

CatalogName
GTR18838871Mouse IGFBP5 ELISA Kit
EK1183Mouse IGFBP5 ELISA Kit PicoKine®
MBS177283-01Mouse IGFBP5 PicoKine ELISA Kit
EK1183-carrier-freeMouse IGFBP5 ELISA Kit PicoKine®, Carrier-free
JOT-EK1500Mo-01Mouse Insulin-like growth factor,IGFBP5 ELISA KIT
E1500Mo-01Mouse Insulin-like growth factor, IGFBP5 ELISA KIT
MBS946276-01Mouse Insulin-like growth factor-binding protein 5, IGFBP5 ELISA Kit
MBS2516043-01Mouse IGFBP5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit
BG-MUS11348Mouse Insulin Like Growth Factor Binding Protein 5 IGFBP5 ELISA Kit
E2986Insulin-Like Growth Factor binding protein 5 (IGFBP5) Mouse ELISA Kit

Popular Products