Mouse IGFBP5 ELISA Kit PicoKine®, Carrier-free

• Background:

  Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.

• :

  heparin or EDTA

• Description:

  Mouse IGFBP5 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Igfbp5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	223	257	257	258	248	14.87	5.9%
  Sample 2	1270	1408	1312	1242	1308	62.88	4.8%
  Sample 3	6078	5394	5607	6353	5858	378.06	6.4%

  *number of samples for each test n=16.

• Synonyms:

  Insulin-like growth factor-binding protein 5;IBP-5;IGF-binding protein 5;IGFBP-5;Igfbp5;Igfbp-5;

• Gene Name:

  Insulin-like growth factor-binding protein 5

• Gene ID:

  16011

• UniProt:

  Q07079

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: L20-E271

• Tissue Specificity:

  Most abundant in kidney, uterus and gastrocnemius muscle.

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  30372 MW

• Specificity:

  Natural and recombinant mouse IGFBP5

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Contains 1 IGFBP N-terminal domain.

• Protein Name:

  Insulin-like growth factor-binding protein 5