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20-Hydroxyecdysone ELISA Kit

CAT: 0247-OKAU00059-5PLATESize: 5 PlateDry Ice: NoHazardous: No
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CAT#:0247-OKAU00059-5PLATESize:5 Plate
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Reproducibility
Sample 20-Hydroxyecdysone Conc. (pg/mL) %CV 1 4,623 8.9 2 2,707 10.6 3 1,997 10.2 Sample 20-Hydroxyecdysone Conc. (pg/mL) %CV Sample 20-Hydroxyecdysone Conc. (pg/mL) %CV 1 4,623 8.9 1 4,623 8.9 2 2,707 10.6 2 2,707 10.6 3 1,997 10.2 3 1,997 10.2 Sample 20-Hydroxyecdysone Conc. (pg/mL) %CV 1 4,522 9.1 2 2,706 9.6 3 1,867 7.8 Sample 20-Hydroxyecdysone Conc. (pg/mL) %CV Sample 20-Hydroxyecdysone Conc. (pg/mL) %CV 1 4,522 9.1 1 4,522 9.1 2 2,706 9.6 2 2,706 9.6 3 1,867 7.8 3 1,867 7.8
CAS Number
7732-18-5
Gene Name
20-Hydroxyecdysone
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
The DetectX® 20-Hydroxyecdysone (20E) Immunoassay kit is designed to quantitatively measure 20E present in extracted tissue samples from hemolymph, plants, or anthropods. Please read the complete kit insert before performing this assay. A 20-hydroxyecdysone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A 20-hydroxyecdysone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to 20-hydroxyecdysone to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound 20-hydroxyecdysone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the 20-hydroxyecdysone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue Extracts
Sensitivity
197.8 pg/mL
Reconstitution
2°C to 8°C
Components
Component Quantity Clear Coated 96 Well Plate 1 or 5 Each 20-Hydroxyecdysone Standard 40 uL or 200 uL DetectX® 20-Hydroxyecdysone Antibody 3 mL or 13 mL DetectX® 20-Hydroxyecdysone Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Clear Coated 96 Well Plate 1 or 5 Each Clear Coated 96 Well Plate 1 or 5 Each 20-Hydroxyecdysone Standard 40 uL or 200 uL 20-Hydroxyecdysone Standard 40 uL or 200 uL DetectX® 20-Hydroxyecdysone Antibody 3 mL or 13 mL DetectX® 20-Hydroxyecdysone Antibody 3 mL or 13 mL DetectX® 20-Hydroxyecdysone Conjugate 3 mL or 13 mL DetectX® 20-Hydroxyecdysone Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each

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