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IL 20 Protein

CAT: 0247-OPPA00641-2UGSize: 2 µgDry Ice: NoHazardous: No
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CAT#:0247-OPPA00641-2UGSize:2 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Gene Aliases
If2d, Zcyto1, Zcyto10
Gene ID
58181
Accession Number
NP_067355.1
Reactivity
Mouse
Target
Interleukin-20 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a total molecular mass of 17,584 Dalton. The IL-20 is purified by proprietary chromatographic techniques.
Type
Protein
Sequence
The sequence of the first five N - terminal amino acids was determined and was found to be Met - Leu - Lys - Thr - Leu.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Bioactivity
The biological activity was determined by measuring the dose dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells) . Significant STAT3 activation is observed with >100 ng/mL of Interleukin-20 recombinant mouse, corresponding to a specific activity of 10,000 units/mg of Interleukin-20 recombinant mouse.
Reconstitution
Reconstitution: //It is recommended to reconstitute the lyophilized Interleukin-20 in sterile 18MΩ -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Storage: //Lyophilized Interleukin-20 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL20 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.Storage: //Lyophilized Interleukin-20 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL20 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Notes
Formerly GWB-BSP054
Protein Length
Recombinant
NCBI Gene Symbol
IL20
Host or Source
E. coli
Protein Name
Interleukin-20
Gene Name URL
IL20

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