Urea Nitrogen Detection Kit

• Reproducibility:

  Sample Urea Nitrogen Conc. (mg/dL) %CV 1 1.24 2.0 2 2.29 1.9 3 4.86 2.8 Sample Urea Nitrogen Conc. (mg/dL) %CV Sample Urea Nitrogen Conc. (mg/dL) %CV 1 1.24 2.0 1 1.24 2.0 2 2.29 1.9 2 2.29 1.9 3 4.86 2.8 3 4.86 2.8 Sample Urea Nitrogen Conc. (mg/dL) %CV 1 1.29 3.1 2 2.35 4.3 3 5.18 3.3 Sample Urea Nitrogen Conc. (mg/dL) %CV Sample Urea Nitrogen Conc. (mg/dL) %CV 1 1.29 3.1 1 1.29 3.1 2 2.35 4.3 2 2.35 4.3 3 5.18 3.3 3 5.18 3.3

• Gene Name:

  Urea Nitrogen

• Type:

  Application Kit

• Assay Principle:

  The DetectX® Urea Nitrogen (also called BUN) Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. Please read the complete kit insert before performing this assay. A urea nitrogen standard calibrated to NIST reference materials is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with Color Reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm. The concentration of urea nitrogen in the sample is calculated, after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of mg/dL urea nitrogen. If samples are to be expressed in terms of mg/dL urea, the data can be converted using the multiplier 2.14.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Urine, Saliva and TCM

• Sensitivity:

  0.030 mg/dL

• Reconstitution:

  RT

• Components:

  Component Quantity Clear 96 Well Plates 2 or 2 by 5 Each Urea Nitrogen Standard 250 uL or 1 mL Color Reagent A 15 mL or 2 by 38 mL Color Reagent B 15 mL or 2 by 38 mL Component Quantity Component Quantity Clear 96 Well Plates 2 or 2 by 5 Each Clear 96 Well Plates 2 or 2 by 5 Each Urea Nitrogen Standard 250 uL or 1 mL Urea Nitrogen Standard 250 uL or 1 mL Color Reagent A 15 mL or 2 by 38 mL Color Reagent A 15 mL or 2 by 38 mL Color Reagent B 15 mL or 2 by 38 mL Color Reagent B 15 mL or 2 by 38 mL