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ACHE ELISA Kit (Rat)

CAT: 0247-OKCA00943-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00943-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Acetylcholinesterase
Reactivity
Rat
Target
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology ACHE ELISA Kit (Rat) (OKCA00943) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for ACHE has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACHE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ACHE captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 95 91-99 EDTA Plasma (n=4) 92 87-96 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95 91-99 Serum (n=5) 95 91-99 EDTA Plasma (n=4) 92 87-96 EDTA Plasma (n=4) 92 87-96
Sensitivity
1.95 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
ACHE
Protein Name
Acetylcholinesterase
Gene Name URL
ACHE

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