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Skin Lysate

CAT: 0247-OCPB00096-100UGSize: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0247-OCPB00096-100UGSize:0.1 mg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reactivity
Human
Type
Cell Lysate
Applications
Western blot
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
2 mg/ml
Format
Liquid. Skin lysate is supplied in SDS sample buffer containing 50 mM DTT.
Reconstitution
2°C to 8°C|-80°C
Host or Source
Human
Preparation
Skin tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors) . Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

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Alternative Products

CatalogName
1419Skin Lysate
XBL-10419Fetal Skin Lysate
XBL-10863Skin Cytoplasmic Lysate
GTR19178979Skin Membrane Lysate
MBS151601-01Skin Tumor Lysate
LS780964Tissue Protein Lysate, Skin
MBS8420877-01Mouse Skin Tissue Lysate

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