Notch1 Antibody

9007-83-4

Notch 1

Neurogenic locus notch homolog protein 1, hN1, Translocation associated notch protein, TAN1, TAN-1, Motch A, mT14, p300, Motch, Mis6, Neurogenic locus notch protein homolog, NICD, Notch 1 intracellular domain, Notch gene homolog 1 (Drosophila), Notch homolog 1, translocation-associated (Drosophila), NOTCH Drosophila, homolog of 1, Translocation associated notch homolog, Translocation associated notch protein TAN 1, xotch

18128

NP_032740.3

Human, Mouse, Rat

Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N (TM) and a N-terminal fragment N (EC) . Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT) . This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

S253-32

Unconjugated

Monoclonal Antibody

WB, ICC, IF

Protein G Purified

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

1 mg/ml

WB (1:1000) ; optimal dilutions for assays should be determined by the user.

Liquid.// PBS pH 7.4, 50% glycerol, 0.09% sodium azide

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Store at -20C. Shipping with Blue Ice or 4C.

IgG1

Detects >270kDa. Does not cross-react with Notch 2 or Notch3.

Notch1

Mouse