FEN1 Antibody

• Gene Name :

  Flap structure-specific endonuclease 1

• Gene Aliases :

  MF1, RAD2, FEN-1

• Gene ID :

  2237

• Accession Number :

  NP_004102.1

• Reactivity :

  Human, Mouse

• Immunogen :

  Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.

• Target :

  The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

• Clonality :

  Monoclonal

• Clone :

  40000000

• Type :

  Monoclonal Antibody

• Applications :

  ICC, IF, IP, WB

• Purification :

  Protein G purified

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  1.73 mg/ml

• Format :

  Liquid

• Buffer :

  PBS

• Reconstitution :

  Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

• Fragment :

  IgG1

• NCBI Gene Symbol :

  FEN1

• Host or Source :

  Mouse

• Protein Name :

  Flap endonuclease 1

• Gene Name URL :

  FEN1

• CAS Number :

  9007-83-4

• Nucleotide Accession Number :

  NM_004111.5