Cd3e Antibody

• Gene Name:

  CD3 antigen, epsilon polypeptide

• Gene Aliases:

  AI504783; CD3; CD3epsilon; T3e; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain.

• Gene ID:

  12501

• Accession Number:

  NP_031674.1XP_006510029.1

• Reactivity:

  Mouse

• Immunogen:

  Mouse BM10-37 cytotoxic T lymphocytes

• Target:

  Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell developement (PubMed:19956738, PubMed:24899501) . Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.

• Clonality:

  Monoclonal

• Clone:

  145-2C11

• Conjugation:

  PerCP

• Type:

  Monoclonal Antibody

• Applications:

  Flow cytometry

• Purification:

  Purified by size-exclusion chromatography

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.5 mg/ml

• Format:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Buffer:

  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

• Reconstitution:

  2°C to 8°C

• Fragment:

  IgG

• Specificity:

  The Armenian hamster monoclonal antibody 145-2C11 reacts with mouse CD3 (epsilon subunit) . This antibody is commonly used as a phenotypic marker for mouse T cells.

• NCBI Gene Symbol:

  Cd3e

• Host or Source:

  Hamster

• Protein Name:

  T-cell surface glycoprotein CD3 epsilon chain

• Gene Name URL:

  Cd3e

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_007648.4XM_006509966.2