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CD3E ELISA Kit (Human)

CAT: 0247-OKEH01335-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01335-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
CD3e molecule
Gene Aliases
CD3e antigen, epsilon polypeptide (TiT3 complex) ; CD3e molecule, epsilon (CD3-TCR complex) ; CD3-epsilon; IMD18; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE.
Gene ID
916
Accession Number
NP_000724.1
Reactivity
Homo sapiens, Human
Target
T-cell surface glycoprotein CD3 epsilon chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD3E ELISA Kit (Human) (OKEH01335) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD3E has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD3E is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD3E captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.23 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
23 kDa
Notes
Formerly GWB-HD6E6H
Specificity
Natural and recombinant Human T-cell surface glycoprotein CD3 epsilon chain
Protein Length
207
NCBI Gene Symbol
CD3E
Protein Name
P07766
Gene Name URL
CD3E
Nucleotide Accession Number
NM_000733.3

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