Cd3e ELISA Kit (Mouse)

CAT: 0247-OKEH04728-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04728-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
CD3 antigen, epsilon polypeptide
Gene Aliases
AI504783; CD3; CD3epsilon; T3e; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain.
Gene ID
12501
Accession Number
NP_031674.1
Reactivity
Mouse, Mus musculus
Target
T-cell surface glycoprotein CD3 epsilon chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cd3e ELISA Kit (Mouse) (OKEH04728) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd3e has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cd3e is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd3e captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
1.56-100ng/mL
Sensitivity
0.88 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
21 kDa
Specificity
Natural and recombinant Mouse T-cell surface glycoprotein CD3 epsilon chain
Protein Length
189
NCBI Gene Symbol
Cd3e
Protein Name
P22646
Gene Name URL
Cd3e
Nucleotide Accession Number
NM_007648.4

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