MITF Antibody

• CAS Number :

  9007-83-4

• Gene Name :

  Melanocyte inducing transcription factor

• Gene Aliases :

  BHLHe32; class E basic helix-loop-helix protein 32; CMM8; COMMAD; melanogenesis associated transcription factor; MI; microphtalmia-associated transcription factor; microphthalmia-associated transcription factor; MITF-A; WS2; WS2A.

• Gene ID :

  4286

• Swiss Prot :

  O75030

• Accession Number :

  NP_000239.1

• Reactivity :

  Human|Mouse

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human MITF.

• Target :

  Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR) . Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) . Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

• Clonality :

  Polyclonal

• Type :

  Polyclonal Antibody

• Applications :

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

• Purification :

  The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  1 mg/ml

• Dilution :

  The optimal working dilution should be determined by the end user.

• Format :

  Liquid.// PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCI, 0.02% sodium azide and 50% glycerol.

• Reconstitution :

  -20°C

• Molecular Weight :

  58 kDa

• Notes :

  Formerly GWB-ASC205

• Fragment :

  IgG

• Specificity :

  MITF Antibody detects endogenous levels of total MITF protein.

• NCBI Gene Symbol :

  MITF

• Host or Source :

  Rabbit

• Protein Name :

  Microphthalmia-associated transcription factor

• Gene Name URL :

  MITF