MITF ELISA Kit (Human)

CAT: 0247-OKEH04343-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04343-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Melanocyte inducing transcription factor
Gene Aliases
BHLHe32; class E basic helix-loop-helix protein 32; CMM8; COMMAD; melanogenesis associated transcription factor; MI; microphtalmia-associated transcription factor; microphthalmia-associated transcription factor; MITF-A; WS2; WS2A.
Gene ID
4286
Accession Number
NP_000239.1
Reactivity
Homo sapiens, Human
Target
Microphthalmia-associated transcription factor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MITF ELISA Kit (Human) (OKEH04343) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MITF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for MITF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MITF captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.35 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
59 KDa
Specificity
Natural and recombinant Human Microphthalmia-associated transcription factor
Protein Length
526
NCBI Gene Symbol
MITF
Protein Name
O75030
Gene Name URL
MITF
Nucleotide Accession Number
NM_000248.3

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