MITF ELISA Kit (Human)

• Reproducibility:

  Mean Inter-assay CV%:

• Gene Name:

  Melanocyte inducing transcription factor

• Gene Aliases:

  BHLHe32; class E basic helix-loop-helix protein 32; CMM8; COMMAD; melanogenesis associated transcription factor; MI; microphtalmia-associated transcription factor; microphthalmia-associated transcription factor; MITF-A; WS2; WS2A.

• Gene ID:

  4286

• Accession Number:

  NP_000239.1

• Reactivity:

  Homo sapiens, Human

• Target:

  Microphthalmia-associated transcription factor

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology MITF ELISA Kit (Human) (OKEH04343) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MITF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for MITF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MITF captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

• Detection Range:

  0.625-40ng/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

• Sensitivity:

  0.35 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  59 KDa

• Specificity:

  Natural and recombinant Human Microphthalmia-associated transcription factor

• Protein Length:

  526

• NCBI Gene Symbol:

  MITF

• Protein Name:

  O75030

• Gene Name URL:

  MITF

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_000248.3