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TFRC Antibody (Phospho-Ser24)

CAT: 0247-OAAF00177-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OAAF00177-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
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CAS Number
9007-83-4
Gene Name
Myelin basic protein|transferrin receptor
Gene Aliases
CD71; Golli-MBP; IMD46; myelin A1 protein; myelin basic protein; myelin membrane encephalitogenic protein; p90; proline-rich proteoglycan 2; T9; TFR; TFR1; TR; transferrin receptor protein 1; TRFR.
Gene ID
4155|7037
Swiss Prot
P02786
Accession Number
NP_001121620.1
Reactivity
Human|Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human CD71/TfR around the phosphorylation site of Ser24.
Target
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity) . A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
84 kDa
Notes
Formerly GWB-ASB380
Specificity
CD71/TfR (Phospho-Ser24) antibody detects endogenous levels of CD71/TfR only when phosphorylated at serine 24.
NCBI Gene Symbol
MBP|TFRC
Host or Source
Rabbit
Protein Name
Transferrin receptor protein 1
Gene Name URL
MBP|TFRC