EIF5B antibody - middle region (ARP61644_P050)

• Gene Name:

  Eukaryotic translation initiation factor 5B

• Gene Aliases:

  IF2

• Gene ID:

  9669

• Swiss Prot:

  O60841

• Accession Number:

  NP_056988

• Reactivity:

  Human, Cow, Dog, Horse, Pig, Rabbit

• Target:

  Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3) . Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

• Partner Proteins:

  TP53; TFCP2; UBC; LGR4; YBX3; ETS1; MMS19; FN1; CSNK2A1; PAXIP1; ECT2; BRCA1; EIF3C; CAND1; YWHAZ; SIRT7; HDGF; Mapk13; POU5F1; ZBTB3; PRKAA1; EIF1AX; CBL; gag

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Sequence:

  SEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAY

• Applications:

  WB

• Purification:

  Affinity Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.5 mg/ml

• Homology:

  Cow: 86%; Dog: 86%; Horse: 86%; Human: 100%; Pig: 93%; Rabbit: 93%

• Format:

  Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Reconstitution:

  For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

• Molecular Weight:

  139kDa

• Protein Length:

  1220

• NCBI Gene Symbol:

  EIF5B

• Host or Source:

  Rabbit

• Protein Name:

  Eukaryotic translation initiation factor 5B

• Gene Name URL:

  EIF5B

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_015904