EIF5B Antibody

• Gene Name :

  Eukaryotic translation initiation factor 5B

• Gene Aliases :

  EIF-5B; eukaryotic translation initiation factor 5B; IF2; translation initiation factor IF2; translation initiation factor IF-2.

• Gene ID :

  9669

• Swiss Prot :

  O60841

• Accession Number :

  NP_056988.3

• Reactivity :

  Human|Mouse|Rat

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human EIF5B.

• Target :

  Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.

• Clonality :

  Polyclonal

• Type :

  Polyclonal Antibody

• Applications :

  Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

• Purification :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution :

  The optimal working dilution should be determined by the end user.

• Format :

  Liquid

• Reconstitution :

  -20°C

• Molecular Weight :

  138 kDa

• Notes :

  Formerly GWB-ASE595

• Specificity :

  EIF5B antibody detects endogenous levels of total EIF5B protein.

• NCBI Gene Symbol :

  EIF5B

• Host or Source :

  Rabbit

• Protein Name :

  Eukaryotic translation initiation factor 5B

• Gene Name URL :

  EIF5B

• CAS Number :

  9007-83-4