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Total Aflatoxin ELISA Kit

CAT: 0026-E4747-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-E4747-100Size:100 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This Total Aflatoxin ELISA Kit is based on the Competitive ELISA method. It can detect Aflatoxin (AF) in samples, such as grain, peanut, formula feed, etc. The microplate provided in this kit has been pre-coated with coupled antigen. During the reaction, AF in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AF antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of AF. The concentration of AF in the samples can be calculated by comparing the OD of the samples to the standard curve.
CAS Number
7732-18-5
Reactivity
Universal
Applications
Sensitivity: 0.02 ppb (ng/ml), Sample recovery rate: Grain/Formula feed- 85%±15%, Peanut- 82%±15%, Edible oil- 85%±15%, Sauce/Wheat/Barley feed- 83%±15%, Beer- 84%±15%, Wine/Soy sauce/Vinegar- 87%±15%, Detection limit: Grain- 0.1 ppb; Formula feed- 0.2 ppb; Edible oil/Peanut- 0.2 ppb; Sauce/Wheat/Barley feed- 0.2 ppb; beer- 0.2 ppb; Wine/Soy sauce/Vinegar- 0.1 ppb, quantitative measurement of Total Aflatoxin in grain and feed samples., Cross-reactivity: Aflatoxin B1 (AFB1) - 100%, AflatoxinB2 (AFB2) - 80%, AflatoxinG1 (AFG1)- 75%, AflatoxinG2 (AFG2) - 45%, FlatoxinM1 (AFM1) - 8%
Detection Method
Absorbance (450 nm)
Features & Benefits
Cross-reactivity: Aflatoxin B1 (AFB1) - 100%, AflatoxinB2 (AFB2) - 80%, AflatoxinG1 (AFG1)- 75%, AflatoxinG2 (AFG2) - 45%, FlatoxinM1 (AFM1) - 8%, Quantitative measurement of Total Aflatoxin in grain and feed samples., Detection limit: Grain- 0.1 ppb; Formula feed- 0.2 ppb; Edible oil/Peanut- 0.2 ppb; Sauce/Wheat/Barley feed- 0.2 ppb; beer- 0.2 ppb; Wine/Soy sauce/Vinegar- 0.1 ppb, Sensitivity: 0.02 ppb (ng/ml), Sample recovery rate: Grain/Formula feed- 85%±15%, Peanut- 82%±15%, Edible oil- 85%±15%, Sauce/Wheat/Barley feed- 83%±15%, Beer- 84%±15%, Wine/Soy sauce/Vinegar- 87%±15%
Shipping Conditions
Gel Pack
Storage Conditions
4°C
Shelf Life
12 months

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