Cholesterol Efflux Fluorometric Assay Kit (cell-based)

Cholesterol Efflux Fluorometric Assay Kit (cell-based)

• Description: Cholesterol efflux from the peripheral tissues and cells in atherosclerotic plaque is an initial and critical step in Reverse Cholesterol Transport (RCT). RCT is the process by which extrahepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, transport excessive cholesterol back to the liver for bile acid synthesis and excretion, thus lowering the peripheral lipid burden. A negative correlation has been established between the in vitro efflux of cholesterol from macrophages and atherosclerosis. BioVision’s Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive, and reproducible method for measuring cholesterol efflux.
• Applications: • Screen serum samples or lipoproteins (isolated or recombinant) for cholesterol efflux. • Screen small molecules for their effect on cholesterol efflux (a valuable tool for drug discovery program).
• Detection Method: Fluorescence (Ex/Em = 482/515 nm).
• Features & Benefits: • Simple & Rapid Protocol • Convenient: Non-Radioactive, no special handling or disposal required • High-Throughput • Accurate: reproducible results with low intra & inter assay variability
• Shipping Conditions: gel pack
• Storage Conditions: -20°C
• Shelf Life: 12 months