CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)

CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)

• Description: Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.
• Features & Benefits: • Simple one-step procedure; takes only ~3 hours • Fast and convenient
• Shipping Conditions: gel pack
• Storage Conditions: -20°C
• Shelf Life: 6-12 months