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CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)

CAT: 0026-K163-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K163-100Size:100 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.
Features & Benefits
• Simple one-step procedure; takes only ~3 hours • Fast and convenient
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
6-12 months