Products for Research Use Only
RegisterLogin

Caspase-3 Fluorometric Assay Kit

CAT: 0026-K105-200Size: 200 AssaysDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0026-K105-200Size:200 Assays
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity.
Features & Benefits
• Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
12 months

Related Products

CatalogName
K124-100Caspase-10 Fluorometric Assay Kit
K114-200Caspase-6 Fluorometric Assay Kit
K110-25Caspase-1 Fluorometric Assay Kit
K139-100Caspase-12 Fluorometric Assay Kit
K126-200Caspase-4 Fluorometric Assay Kit
K122-25Caspase-5 Fluorometric Assay Kit
K112-100Caspase-8 Fluorometric Assay Kit
K118-400Caspase-9 Fluorometric Assay Kit
K114-100Caspase-6 Fluorometric Assay Kit
K116-25Caspase-2 Fluorometric Assay Kit

Alternative Products

CatalogName
K2007-25Caspase-3 Fluorometric Assay Kit
55R-1269Caspase 3 Fluorometric Assay Kit
GR107006Genorise® Caspase-3 Fluorometric Assay Kit
TBS2031FCaspase-2 Fluorometric Assay Kit
TBS2034FCaspase-6 Fluorometric Assay Kit
TBS2036FCaspase-7 Fluorometric Assay Kit
TBS2041FCaspase-12 Fluorometric Assay Kit
TBS2055Caspase-Family Fluorometric Assay kit
TBS2037FCaspase-8 Fluorometric Assay Kit
TBS2045Caspase-1 Fluorometric Assay kit

Popular Products