Caspase-10 Fluorometric Assay Kit

• Description:

  Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-10 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence AEVD. The assay is based on detection of cleavage of substrate AEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). AEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-10 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.

• Features & Benefits:

  • Simple one-step procedure; takes only 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in AEVD-dependent caspase activity.

• Shipping Conditions:

  gel pack

• Storage Conditions:

  -20°C

• Shelf Life:

  6-12 months