Caspase-6 Fluorometric Assay Kit

CAT:

26-K114-100

Size:

100 Assays

Price:

Ask

For price, please contact [email protected]

Description: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-6 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on detection of cleavage of substrate VEID-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VEID-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by Mch2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
Features & Benefits: • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-6 activity.
Shipping Conditions: gel pack
Storage Conditions: -20°C
Shelf Life: 12 months

Cat	Name
K114-100	Caspase-6 Fluorometric Assay Kit
K114-25	Caspase-6 Fluorometric Assay Kit
K114-400	Caspase-6 Fluorometric Assay Kit
K114-200	Caspase-6 Fluorometric Assay Kit
K105-100	Caspase-3 Fluorometric Assay Kit
K112-25	Caspase-8 Fluorometric Assay Kit
K116-100	Caspase-2 Fluorometric Assay Kit
K122-25	Caspase-5 Fluorometric Assay Kit
K122-400	Caspase-5 Fluorometric Assay Kit
K124-25	Caspase-10 Fluorometric Assay Kit

Cat	Name
55R-1276	Caspase 6 Fluorometric Assay Kit
K2014-100	Caspase-6 Fluorometric Assay Kit
K2014-200	Caspase-6 Fluorometric Assay Kit
K2014-400	Caspase-6 Fluorometric Assay Kit
K2014-25	Caspase-6 Fluorometric Assay Kit
TBS2034F	Caspase-6 Fluorometric Assay Kit
GWB-AXR040	Caspase-6 Fluorometric Assay Kit - 100 assays
GWB-AXR042	Caspase-6 Fluorometric Assay Kit - 25 assays
GWB-AXR043	Caspase-6 Fluorometric Assay Kit - 400 assays
GWB-AXR041	Caspase-6 Fluorometric Assay Kit - 200 assays