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Caspase-2 Fluorometric Assay Kit

CAT: 0026-K116-25Size: 25 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K116-25Size:25 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
Features & Benefits
• Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity.
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
12 months

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