Products for Research Use Only
RegisterLogin

CD45 Antibody Cocktail

CAT: 0013-GTR18572063Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18572063Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
This antibody cocktail contains an antibody which binds to all CD45 leukocyte common antigen (LCA) family isoforms (clone 2B11), and an antibody specific to the CD45RB isoform (clone PD7/26) . CD45 is expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of the CD45 isoforms have protein phosphatase activity and are involved in regulation of transmembrane signals. antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this antibody cocktail is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody cocktail detects CD45 expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
Reactivity
Human
Immunogen
Isolated neoplastic cells from T cell lymphoma were used as the immunogen for clone 2B11 and human peripheral blood lymphocytes maintained in Tcell growth factor were used as the immunogen for clone PD7/26.
Clonality
Monoclonal
Clone
2B11 + PD7/26
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 0.5-2ug/10^6 cells, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD 45 antibody cocktail to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

Related Products

CatalogName
GTR18572922CD47 Antibody Cocktail
GTR18082324-01CD47 Antibody Cocktail
GTR18573512MITF Antibody Cocktail
GTR18573282PAX8 Antibody Cocktail
GTR18573097Myogenin Antibody Cocktail
GTR18572605SMMHC Antibody Cocktail
GTR18572397CD57 Antibody Cocktail
GTR18572387GFAP Antibody Cocktail
GTR18572386GFAP Antibody Cocktail
GTR18572177Chromogranin A Antibody Cocktail

Alternative Products

CatalogName
V2243-20UGCD45 Antibody Cocktail
V7330-100UGRecombinant CD45 Antibody Cocktail
GT14909Anti-human CD3 FITC/CD4 PE/CD45 PerCP Cocktail
V3139-20UGMyogenin Antibody Cocktail
V2027IHC-7MLCD1a Antibody Cocktail
V3156SAF-100UGMelan-A Antibody Cocktail
V2694SAF-100UGEpCAM Antibody Cocktail
V2455IHC-7MLChromogranin A Antibody Cocktail
V2937-20UGZAP70 Antibody Cocktail
V2752SAF-100UGSMMHC Antibody Cocktail

Popular Products