Recombinant Vimentin Antibody

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

P08670

Human

Recombinant full-length human protein was used as the immunogen for the recombinant Vimentin antibody.

VIM/6576R

Unconjugated

Musculoskeletal & Connective Tissue Research

Protein A/G affinity

Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

For research use only.

Optimal dilution of the recombinant Vimentin antibody should be determined by the researcher.

IHC-P, WB

Rabbit

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide