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Recombinant Golgi Antibody

CAT: 0013-GTR18575401Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18575401Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.
UniProt
Q92896
Reactivity
Human
Immunogen
Full length recombinant GLG1 protein was used as the immunogen for this recombinant Golgi antibody.
Clonality
Recombinant
Clone
GLG1/2829R
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein A affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the recombinant Golgi antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG, kappa

UniProtKB · Q92896

Golgi apparatus protein 1

GSLG1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q92896
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GLG1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B7Z8Y4, D3DUJ7, Q13221, Q6P9D1
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmCytoskeletonGolgi apparatusMembrane

PTM

GlycoproteinPhosphoprotein

Technical term

Proteomics identificationReference proteome

Domain

RepeatSignalTransmembraneTransmembrane helix

Ligand

Sialic acid

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