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Recombinant Dog Anionic trypsin

CAT: 0399-CSB-EP356954DO-02Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-EP356954DO-02Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
; Anionic trypsin; EC 3.4.21.4
Abbreviation
Recombinant Dog Anionic trypsin protein
UniProt
P06872
Expression Region
24-247aa
Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Target Sequence
IVGGYTCEENSVPYQVSLNAGYHFCGGSLISDQWVVSAAHCYKSRIQVRLGEYNIDVLEGNEQFINSAKVIRHPNYNSWILDNDIMLIKLSSPAVLNARVATISLPRACAAPGTQCLISGWGNTLSSGTNYPELLQCLDAPILTQAQCEASYPGQITENMICAGFLEGGKDSCQGDSGGPVVCNGELQGIVSWGYGCAQKNKPGVYTKVCNFVDWIQSTIAANS
Tag
N-terminal 6xHis-SUMO-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Others
Relevance
Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
40 kDa
References & Citations
"Differential regulation of trypsinogen mRNA translation: full-length mRNA sequences encoding two oppositely charged trypsinogen isoenzymes in the dog pancreas." Pinsky S.D., Laforge K.S., Scheele G. Mol. Cell. Biol. 5:2669-2676 (1985)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein

UniProtKB · P06872

Anionic trypsin

TRY2_CANLF · Canis lupus familiaris

View on UniProt ↗
Primary accession
P06872
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
—
Protein existence
2: Evidence at transcript level
Organism
Canis lupus familiaris (Dog)
Taxonomy ID
9615
Alternative names
—
EC number
3.4.21.4
Processing
Precursor
Secondary accessions
—
Protein keywords

Ligand

CalciumMetal-binding

Biological process

Digestion

PTM

Disulfide bondZymogen

Molecular function

HydrolaseProteaseSerine protease

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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