Universal 3' miRNA Reverse Primer

Availability

Please contact us every day between 9am-6pm local time to check the availability and/or to request a quote. For larger quantities a discount may apply.
Ordering

If you wish to order this product, please include the catalogue number #MPH00000 in your purchase order among with your shipping and billing information.
Technical file

Please contact us to request the latest datasheet, protocol, certificate of analysis and SDS files.
Notes

Designed for Research Use Only. Manufactured in Canada.
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Description

Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
Goup

reverse transcription

Name

Reverse Transcriptase Polymerase Chain Reaction
Concept

Scope note: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Tree numbers
- E05.393.620.500.725
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data