Universal cDNA Reverse Transcribed by Random Hexamer: Chicken Normal Tissues
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Catalog number
C4C34565-R
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Price
Please ask
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Size
100 rxn
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Shipping conditions
Dry Ice
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Category
DNA
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SubCategory
Universal cDNA
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Description
this 1 cDNA is double stranded complementary DNA derived by BioChain from mRNA with very high fidelity reverse transcription using a pfu or pfx mix, Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
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Goup
reverse transcription
MeSH Data
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Name
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Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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