Universal cDNA Reverse Transcribed by Oligo dT Primer: Human Normal Tissues
-
Catalog number
C4234565
-
Price
Please ask
-
Size
100 rxn
-
-
Shipping conditions
Dry Ice
-
Category
DNA
-
SubCategory
Universal cDNA
-
Description
this 1 cDNA is double stranded complementary DNA derived by BioChain from mRNA with very high fidelity reverse transcription using a pfu or pfx mix, Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Goup
reverse transcription
-
Gene target
-
Short name
Universal cDNA Reverse Transcribed by Oligo dT Primer: Normal Tissues
-
Technique
reverse, cDNA, cDNAs
-
Species
Human, Humans
-
Alternative name
Universal complementary Desoxyribonucleic acid Reverse Transcribed by Oligo dT Primer: H. sapiens healthy Tissues
-
Alternative technique
transctription, pcr-sequence, pcr
-
Tissue
cdna, normal
-
MeSH Data
-
Name
-
Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
-
Tree numbers
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products