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Long name
Rabbit Polyclonal Antibody against Mouse PAG / Cbp, Clone: PAb
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Antigen
PAG / Cbp
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Species reactivity
Mouse (Mus musculus), Human (Homo sapiens)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Polyclonal Antibody (PAb)
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Clone
Polyclonal
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Applications
IP, WB, IHC(P)
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Immunogen
Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).
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Format conjugation
purified; unconjugated
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Specificity
The polyclonal antibody recognizes Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
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Research area
Adaptor Proteins (Rodent), Adaptor Proteins (Human), Adaptor Proteins
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Rabbit Polyclonal Antibody against Mouse PAG / Cbp, Clone: PAb is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified from rabbit serum by precipitation methods
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References
Contact us for the most recent list of references
