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Long name
Mouse Monoclonal Antibody against Mouse PAG / Cbp, Clone: PAG-C1
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Antigen
PAG / Cbp
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Species reactivity
Mouse (Mus musculus), Human (Homo sapiens), Rat, Bovine, Other not tested
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
PAG-C1
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Applications
IP, WB, IHC(P)
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Immunogen
C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
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Format conjugation
purified; unconjugated
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Specificity
The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
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Research area
Adaptor Proteins (Veterinary), Adaptor Proteins (Rodent), Adaptor Proteins (Human), Adaptor Proteins
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Mouse PAG / Cbp, Clone: PAG-C1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Vang T, Abrahamsen H, Myklebust S, Horejsi V, Tasken K: Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling. J Biol Chem. 2003 May 16;278(20):17597-600. , *Davidson D, Bakinowski M, Thomas ML, Horejsi V, Veillette A: Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor._x000D_ Mol Cell Biol. 2003 Mar;23(6):2017-28. _x000D_ , *Baumgartner M, Angelisova P, Setterblad N, Mooney N, Werling D, Horejsi V, Langsley G: Constitutive exclusion of Csk from Hck-positive membrane microdomains permits_x000D_ Src kinase-dependent proliferation of Theileria-transformed B lymphocytes. Blood. 2003 Mar 1;101(5):1874-81. _x000D_
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
