Mouse Anti-FLAG Tag Monoclonal Antibody
#
-
Catalog numberAKR-004
-
Price:
-
Size100 µg
-
-
We send this reagant onBlue Ice
-
The temperatureerature for storage is-20ºC
-
SDS or safty data sheetmore (AKR-004) GHS.pdf
-
Type of reagentCell Signaling and Protein Biology
-
Related techniquesCell Signaling and Protein Biology, Epitope Tags, Epitope Tag Antibodies
-
Use of the reagentMouse Anti-FLAG Tag Monoclonal Antibody is Protein A purified and provided at 1 mg/mL. Suitable for Western blot or Immunostaining. 100 µg.
-
Cellular usePurified antibody is provided at a concentration of 1 mg/mL
-
Host speciesMouse
-
Isotype controlIgG2b
-
Conjugation and formUnconjugated
-
Species compatible toN/A
-
Use of this test kit or productIB, IS, IP, ELISA, Dot Blot
-
AntigenDYKDDDDK synthetic peptide conjugated to KLH
-
Buffer100 μg of Protein A purified antibody at 1 mg/mL in 10 mM PBS, pH 7.2, containing 0.05% NaN3
-
Molecular weightVaries
-
This reagent is purifiedProtein A
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
PropertiesIf you buy Antibodies supplied by Cellular biology labs they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
-
ConjugationFlag
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
French translationanticorps
-
Gene target
-
Short nameMouse Anti- Tag Monoclonal Antibody
-
TechniqueAntibody, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, Tags
-
Hostmouse
-
LabelFlag
-
SpeciesMouse, Mouses
-
Alternative nameMouse Antibody toFLAG detection labelled monoclonal (antibody to-)
-
Alternative techniqueantibodies, murine
-
Clone nameFG4R
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data