FITC-Conjugated Rat IgG1 Isotype Control
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Catalog number
RICIGG1-02MG
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Price
Please ask
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Size
200 µg
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Clone
RTK2071
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Conjugated
FITC
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Tested application
Flow Cytometry
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Long description
This FITC-Conjugated Rat IgG1 Isotype Control was affinity purified from rabbit serum. It does not cross-react with any human or animal antigen
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Storage buffer
PBS, 0,09% sodium Azide, may contain carrier protein-stabilizer
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Shipping conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Technical datasheet
Please contact us to receive the latest datasheet, the certificate of analysis or the MSDS
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Regulation status
This antibody is intended for Research Use Only.
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
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Properties
This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Conjugation
Anti-FITC Antibody
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Group
isotypes
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Short name
FITC-Conjugated IgG1 Isotype Control
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Technique
Control, Isotype, FITC, antibody Conjugates, controls, Fluorescein, Isotypes and controls
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Host
Rat
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Isotype
IgG1, IgG1
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Label
FITC
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Species
Rat, Rats
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Alternative name
fluorecein-coupled Rat IgG1 isoform reference
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Alternative technique
control, controls, fluorescine
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Tissue
control
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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