Rat IgG1 Isotype Control (Clone: HRPN)- FITC
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Catalog number
MBS668904
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Price
Please ask
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Size
0.1 mg
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Products_type
Isotype Control
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Products_short_name
[IgG1]
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Products_gene_name
[IgG1]
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Clonality
Monoclonal
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Clone
[HRPN]
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Purity
Purified Affinity Chromatography
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Form
0.2 mg/ml in Tris buffer containing 0.05% Azide
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Storage_stability
Store the antibody at 4 degree C, stable for 6 months.
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Tested_application
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Conjugation
Anti-FITC Antibody
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Group
isotypes
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Short name
IgG1 Isotype Control (Clone: HRPN)- FITC
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Technique
Control, Isotype, FITC, controls, Fluorescein, Isotypes and controls
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Host
Rat
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Isotype
IgG1, IgG1
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Label
FITC
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Species
Rat, Rats
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Alternative name
Rat IgG1 isoform reference (clonality: HRPN)- fluorecein
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Alternative technique
control, controls, fluorescine
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Tissue
control
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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