IgG1 Isotype Control Fc fusion protein biotin
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Catalog number
35R-0022
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Price
Please ask
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Size
100 µg
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Category
Biological Reagents
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Antibody Subtype
Isotype Controls
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Area of research
Immunology
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Raised in
Rat
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Isotypes
IgG1 kappa
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Concentration
500 ug/ml
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Form Buffer
Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
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Storage
Store at 2-8 deg C. Do not freeze. Protect from light.
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Shipping conditions
Blue Ice
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Tested for
FC
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Usage Recommendations
FC: ≤ 0.125 ug/test
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Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Conjugation
Biotinylated
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.
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Group
isotypes
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Gene target
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Short name
IgG1 Isotype Control Fc protein
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Technique
Control, Isotype, FC, controls, Isotypes and controls
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Isotype
IgG1
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Label
Biotin
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Alternative name
Rat monoclonal IgG1 Isotype Control Fc fusion protein (biotin)
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Alternative technique
control, controls
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Tissue
control
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