Proteinase K, From Pichia pastoris, Recombinant PCR grade
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Catalog number
RM2957-20X10MG
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Price
Please ask
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Size
20X10MG
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Description
Proteinase K, From Pichia pastoris, Recombinant PCR grade is a white to off-white lyophilized powder. This product is useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
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Shelf life
3 years
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Storage
Store at 2 - 8°C
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Synonym
Endopeptidase K
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Molecular Formula
NA
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Molecular Weight
28.93
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Appearance
White to off-white lyophilized powder
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Uses
Laboratory chemicals, Manufacture of substances
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Personal Protection
Wear respiratory protection. Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.
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Toxicological Information
NA
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Ordering
To order Proteinase K, From Pichia pastoris, Recombinant PCR grade, please use the Cat. Nr.RM2957-20X10MG and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Proteinase K, From Pichia pastoris, Recombinant PCR grade
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Group
PCR, polymerase chain reaction, recombinants
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene
Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
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Source
Recombinants or rec. proteins
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Gene target
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Short name
Proteinase K, From Pichia pastoris, Recombinant PCR grade
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Technique
Recombinant, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Himedia advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
Proteinase K, From Pichia pastoris, Rec. PCR test kit grade
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Alternative technique
rec, dna-amplification
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MeSH Data
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Name
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Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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