Proteinase K ex. Pichia Pastoris (Type D - Recombinant) for molecular biology & PCR

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• Catalog number
  32753
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• Size
  500 mg

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• Reagent properties
  Intermediates and solvents for biotechnological research
• Chemical CAS Nr
  39450-01-6
• Group
  PCR, polymerase chain reaction, recombinants
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
• Gene
  Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
• Source
  Recombinants or rec. proteins

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• Gene target
  Proteinase   Pichia   Pastoris   for   molecular   biology  
• Short name
  Proteinase K ex. Pichia Pastoris ( D - Recombinant) for molecular biology & PCR
• Technique
  Recombinant, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Research sys advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Proteinase K ex. Pichia Pastoris (classification D - Rec.) to measure molecular biology & PCR test kit
• Alternative technique
  rec, dna-amplification

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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