Proteinase K, From Pichia pastoris, Recombinant PCR grade
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Catalog numberRM2957-1X1G
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Price:
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Size1X1G
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DescriptionProteinase K, From Pichia pastoris, Recombinant PCR grade is a white to off-white lyophilized powder. This product is useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
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Shelf life3 years
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StorageStore at 2 - 8°C
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SynonymEndopeptidase K
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Molecular FormulaNA
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Molecular Weight28.93
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AppearanceWhite to off-white lyophilized powder
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UsesLaboratory chemicals, Manufacture of substances
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Personal ProtectionWear respiratory protection. Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.
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Toxicological InformationNA
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OrderingTo order Proteinase K, From Pichia pastoris, Recombinant PCR grade, please use the Cat. Nr.RM2957-1X1G and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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TipsOur specialists recommend you to follow carefully the pre-registered instructions for Proteinase K, From Pichia pastoris, Recombinant PCR grade
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GroupPCR, polymerase chain reaction, recombinants
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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GeneProteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
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SourceRecombinants or rec. proteins
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Gene target
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Short nameProteinase K, From Pichia pastoris, Recombinant PCR grade
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TechniqueRecombinant, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Himedia advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative nameProteinase K, From Pichia pastoris, Rec. PCR test kit grade
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Alternative techniquerec, dna-amplification
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MeSH Data
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Name
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ConceptScope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
- E05.393.620
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data