CD3-FITC/ CD16+56-PE/ CD45-PECy5, Mouse Monoclonal antibody-Human, Clone: Cris-7/ LD16/ C5.9/ J.33
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Catalog numberACYT3F216PE656PE45C2
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PricePlease ask
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Size1.5 ml
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Alternative name 1CD3-FITC/ CD16+56-PE/ CD45-PECy5, Mab anti-Human, Clone: Cris-7/ LD16/ C5.9/ J.33
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Alternative name 2CD3-FITC/ CD16+56-PE/ CD45-PECy5, Mab antibody-Human, Clone: Cris-7/ LD16/ C5.9/ J.33
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Alternative name 3CD3-FITC/ CD16+56-PE/ CD45-PECy5, Mab antibody-Homo sapiens, Clone: Cris-7/ LD16/ C5.9/ J.33
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Alternative name 4anti-CD3-CD16+56-CD45- Clone: Cris-7/ LD16/ C5.9/ J.33
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Alternative name 5CD3-CD16+56-CD45- Clone: Cris-7/ LD16/ C5.9/ J.33
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolPTPRCAP, PTPRC, IGLVI-56, IGHV7-56, ETV3, ERF, PARVB, RNU6-56P, TRIM68, FCGR3A
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Short nameCD3-FITC/ CD16+56-PE/ CD45-PECy5, Mouse Monoclonal antibody- , Clone: Cris-7/ LD16/ C5 9/ J 33
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TechniqueMouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameCD3-fluorecein/ CD16and56-peroxidase/ CD45-PECy5, Mouse monoclonal (antibody to-)-H. sapiens, clonality: Cris-7/ LD16/ complement component 5.9/ J.33
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Alternative techniqueantibodies, murine, fluorescine
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Alternative to gene targetcomplement component 5, C5a and C5b and C5D and CPAMD4 and ECLZB, C5 and IDBG-83167 and ENSG00000106804 and 727, C5a anaphylatoxin chemotactic receptor binding, Extracellular, Hc and IDBG-163835 and ENSMUSG00000026874 and 15139, BT.24055 and IDBG-638834 and ENSBTAG00000012210 and 512045
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Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C associated protein
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Synonyms
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Locus
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Discovery year1996-04-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable (I)-56 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable (I)-56
- immunoglobulin lambda variable (I)-56 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 7-56 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 7-56
- immunoglobulin heavy variable 7-56 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameETS variant transcription factor 3
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Synonyms gene name
- ets variant gene 3, ETS family transcriptional repressor
- ETS variant 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-12-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameETS2 repressor factor
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Synonyms gene name
- Ets2 repressor factor
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameparvin beta
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-04-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Parvins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 56, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 56
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Locus
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Discovery year2011-08-08
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nametripartite motif containing 68
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Synonyms gene
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Synonyms gene name
- ring finger protein 137
- tripartite motif-containing 68
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-05-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ring finger proteins
- Tripartite motif containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameFc gamma receptor IIIa
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Synonyms gene
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Synonyms gene name
- Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
- Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
- Fc fragment of IgG receptor IIIa
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Synonyms
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GenBank acession
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Locus
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Discovery year1988-11-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin like domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data