Calpain Activity Fluorometric Assay Kit

• Catalog number
  K240-100
• Price
  Please ask
• Size
  100 assays

• Description
  Exclusively detects activated calpain
• Summary
  • Detection method- Fluorescence (Ex/Em 400/505 nm) • Species reactivity- Mammalian • Applications- The kit provides a simple and convenient means for analyzing calpain activity in apoptotic and other samples. The assay is based on fluorometric detection of the cleavage of calpain substrate at 505 nm using a fluorometer or a fluorecence plate reader.
• Sample Type
  Cell and tissue lysates
• Features Benefits
  • Simple one-step procedure; takes only ~2 hours • Fast and convenient • The kit contains both positive and negative controls. Active calpain and calpain inhibitor and antibodies are also available separately.
• Storage Conditions
  -70°C
• Shipping Conditions
  dry ice
• Shelf life
  6-12 months
• Background
  Activation of calpain is involved in many forms of physiological and pathological processes (e.g., apoptosis). Calpain activation requires cell membrane and Ca²ᶧ, and activated calpain is released into cytosol. The Calpain Activity Assay Kit provides optimized buffers and reagents for a convenient measurement of calpain activity. The Extraction Buffer provided with the kit specifically extracts cytosolic proteins without contaminations of cell membrane and lysosome proteases. The Extraction Buffer also prevents auto-activation of calpain during the extraction procedure. Thus, the kit detects only activated calpain in cytosol upon treatment of cells with inducers (e.g., chemicals or drugs). The fluorometric assay is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by calpain, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence plate reader. Comparison of the fluorescence intensity from a treated sample with a normal control allows determination of the changes in calpain activity.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Calpain   Activity   Fluorometric   Kit  
• Short name
  Calpain Activity Fluorometric Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Calpain Activity fluorescence test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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