Beta-Secretase Activity Fluorometric Assay Kit

Description

Highly Sensitive Assay
Summary

• Detection method- Fluorescence (Ex/Em 335-355/ 495-510 nm) • Species reactivity- Mammalian • Applications- Convenient fluorescence method for detecting β-secretase activity in biological and purified samples.
Sample Type

Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Features Benefits

• Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable.
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

β-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer’s disease. The β-Secretase activity Assay Kit provides a convenient fluorescence method for detecting β-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.
Data Sheet Link
Test

Biovision supplies other types of Assays as 1.

Gene target

Beta-Secretase Activity Fluorometric Kit
Short name

Beta-Secretase Activity Fluorometric Assay Kit
Technique

Assay, assays
Host

Assay
Alternative name

b-Secretase Activity fluorescence test reagent
Alternative technique

arrays, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832