Mini-blotter for Spoligotyping

• Catalog number
  MN45
• Price
  Please ask
• Size
  1 EA

• Availability
  Please contact Gentaur/Genprice to check the latest availability
• Ordering
  Current price is for Diagnostics labs. Special -19% discount for Research Institutes. For ordering please contact us at +32 16 58 90 45
• Technical file
  Please contact us to request the current datasheet or security datasheet
• Test
  A rapid, polymerase chain reaction (PCR)-based method for genotyping strains of the Mycobacterium tuberculosis complex (MTB). Spoligotyping data can be represented in absolute terms (digitally), and the results can be readily shared among laboratories, thereby enabling the creation of large international databases. Since the spoligotype assay was standardized more than 10 yr. ago, tens of thousands of isolates have been analyzed, giving a global picture of MTB strain diversity. The GENTAUR method is highly reproducible and has been developed into a high-throughput assay for large molecular epidemiology projects. In the United States, spoligotyping is employed on nearly all newly identified culture-positive cases of tuberculosis as part of a national genotyping program. The strengths of this method include its low cost, its digital data results, the good correlation of its results with other genetics markers, its fair level of overall differentiation of strains, its high-throughput capacity, and its ability to provide species information. However, the method's weaknesses include the inability of spoligotyping to differentiate well within large strain families such as the Beijing family, the potential for convergent evolution of patterns, the limited success in improving the assay through expansion, and the difficulty in obtaining the specialized membranes and instrumentation

• Gene target
  Mini-blotter   for  
• Short name
  Mini-blotter for Spoligotyping
• Technique
  Spoligotyping, spoligotypings
• Alternative name
  Mini-blotter to measure Spoligotyping
• Alternative technique
  mycobacteria

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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