Spoligotyping Kit without controls and
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Catalog number
521-IM9702
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Price
Please ask
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Size
1 vial
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Ordering information
The catalog number at Gentaur for this research reagent is 521-IM9702. It is a frequent ordered item.
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Delivery
The Spoligotyping Kit without controls and can be delivered to your laboratory the next week after your order if you order before Friday 15:00.
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Users reference
Contact us for laboratory references of researchers that recently used this product in Europe or the United States.
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Description
Positive controls are often requested for publication of your result. Reference 1 can be used to spike a vast number of experiments
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Test
A rapid, polymerase chain reaction (PCR)-based method for genotyping strains of the Mycobacterium tuberculosis complex (MTB). Spoligotyping data can be represented in absolute terms (digitally), and the results can be readily shared among laboratories, thereby enabling the creation of large international databases. Since the spoligotype assay was standardized more than 10 yr. ago, tens of thousands of isolates have been analyzed, giving a global picture of MTB strain diversity. The GENTAUR method is highly reproducible and has been developed into a high-throughput assay for large molecular epidemiology projects. In the United States, spoligotyping is employed on nearly all newly identified culture-positive cases of tuberculosis as part of a national genotyping program. The strengths of this method include its low cost, its digital data results, the good correlation of its results with other genetics markers, its fair level of overall differentiation of strains, its high-throughput capacity, and its ability to provide species information. However, the method's weaknesses include the inability of spoligotyping to differentiate well within large strain families such as the Beijing family, the potential for convergent evolution of patterns, the limited success in improving the assay through expansion, and the difficulty in obtaining the specialized membranes and instrumentation
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Gene target
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Short name
Spoligotyping Kit without controls
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Technique
Spoligotyping, Controls, control, spoligotypings
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Alternative name
Spoligotyping reagent without controls and
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Alternative technique
mycobacteria, controls, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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