Rat anti Forssman antigen

• Catalog number
  MUB0609S
• Price
  Please ask
• Size
  1 mL

• Category
  Primary Antibodies
• Long description
  The Forssman glycolipid is a glycosylceramide possessing a neutral pentasaccharide head group, referred to as the Forssman antigen (Fa) and is a member of the globoseries glycolipid family. Fa has been identified in a number of mammals and exhibits heterogeneity with respect to developmental and cell-type expression among species. Some studies have reported the presence of the Fa in certain Human embryonic and tumor cells.The majority of Human individuals have undetectable levels of Fa.Gastric and colonic tumors from Fa-negative patients, however, contained relatively high levels of Fa. Moreover, compared with normal lung tissue, many lung cancers contained elevated levels of Fa. These observations arouse interest in Fa for a possible role as a Human tumor antigen.
• Antibody come from
  The antibody 33B12 is a Rat anti-Forrsman glycosphingolipid (FGL) antibody derived by fusing Mouse myeloma cells with spleen cells from a Rat immunized with Mouse mammary tumors.
• Other description
  Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
• Clone
  33B12
• Antigen antibody binding interaction
  Rat anti Forssman antigen Antibody
• Antibody is raised in
  Rat
• Antibody s reacts with
  Mouse
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  Clone 33B12 and 117C9 detect overlapping epitopes on the Forssman glycolipid hapten (GalNA1- c3aG alNAcD1 -3GalaI-4GalB1-4GlcBl-lCer). Clone 33B12 reacts with the terminal sugar sequence GalNAcd-3GalNAc and is specific for Forssman antigen.
• Research interest
  Cancer,Immunology
• Application
  Immunohistochemistry (frozen)
• Antibody s suited for
  33B12 is useful for immubohistochemistry on frozen sections. Optimal antibody dilution should be determined by titration.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Sonnenberg A, van Balen P, Hengeveld T, Kolvenbag GJ, Van Hoeven RP, Hilgers J., Monoclonal antibodies detecting different epitopes on the Forssman glycolipid hapten. J Immunol. 1986 Aug 15;137(4):1264-9
• Protein number
  Q8VI38
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antigens are peptides or recombinant or native dependent on the production method.
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  Forssman  
• Gene symbol
  GBGT1, PHF6
• Short name
  anti Forssman antigen
• Technique
  antigen, anti, antibody to, antigenes
• Host
  Rat
• Isotype
  IgG2c
• Label
  unlabeled
• Species
  Rat, Rats
• Alternative name
  Rat antibody to Forssman protein
• Alternative technique
  antigenes, antibodies

Gene info

• Identity
  HGNC:20460
• Gene
  GBGT1
• Long gene name
  globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 (FORS blood group)
• Synonyms
  • UDP-GalNAc
  • A3GALNT
  • MGC44848
  • FS
• Synonyms name
  • Forssman glycolipid synthetase (FS)
  • Forssman synthetase
  • Forssman blood group
• GenBank acession
  • AY358175
• Locus
  9q34.2
• Discovery year
  2004-05-17
• Entrez gene record
  26301
• Pubmed identfication
  • 10506200
  • 8855242
• RefSeq identity
  • NM_021996
• Classification
  • Blood group antigens
  • Glycosyltransferase family 6
• VEGA ID
  OTTHUMG00000020853
• Locus Specific Databases
  • LRG_826

Gene info

• Identity
  HGNC:18145
• Gene
  PHF6
• Long gene name
  PHD finger protein 6
• Synonyms gene
  • BFLS
  • BORJ
• Synonyms gene name
  • Borjeson-Forssman-Lehmann syndrome
• Synonyms
  • KIAA1823
  • MGC14797
  • CENP-31
• Synonyms name
  • centromere protein 31
• GenBank acession
  • AB058726
• Locus
  Xq26.2
• Discovery year
  2002-02-28
• Entrez gene record
  84295
• Pubmed identfication
  • 12415272
  • 15466013
• RefSeq identity
  • NM_032458
• Classification
  • PHD finger proteins
• VEGA ID
  OTTHUMG00000022453
• Locus Specific Databases
  • LRG_629

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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